论文部分内容阅读
采用内部简单序列重复(ISSR)和随机扩增多态性DNA标记(RAPD)方法鉴定亚麻花药培养中得到的植株是否起源于小孢子。F1供试植株双亲间的多态性片段已被鉴别出来,它们在花药苗中的分离模式被用来分析这些植物的起源和确定由同一愈伤组织分化出的花药苗的同源性。运用一种ISSR引物(UBC889)和两种RAPD引物(UBC556和561)进行鉴定,16个植株中,有12个明显来自于小孢子。来自于相同愈伤组织的植株在5个多态性位点有着相同的PCR模式,因此认为这些植株极有可能来自于相同的小孢子。因此,建议用形成根的愈伤组织的数量来描述亚麻花药培养的效率。
Internal simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) were used to identify whether the plants obtained from flax anther culture originated in microspores. Polymorphic fragments of F1 parents tested have been identified and their patterns of separation in anther shoots were used to analyze the origin of these plants and to determine the identity of anther shoots differentiated from the same callus. An ISSR primer (UBC889) and two RAPD primers (UBC556 and 561) were used for identification, of which 12 of 16 were apparently derived from microspores. Plants from the same callus have the same PCR pattern at the five polymorphic sites and therefore most probably these plants are from the same microspore. Therefore, it is advisable to describe the efficiency of flax anther culture using the number of callus forming roots.