Construction and Biological Function Analysis of a dldh Deletion Mutant Strain of Vibrio alginolytic

• 作 者: Huanying PANG
• 机 构: FisheriesCollege,GuangdongProvincialKeyLaboratoryofPathogenicBiologyandEpidemiologyforAquaticEconomi
• 出 处: 农业生物技术：英文版
• 发表日期: 2015年4期
• 关键词: 缺失突变株 生物学功能 溶藻弧菌 游泳能力 重叠PCR 斜带石斑鱼 致病性 重组技术 Vibrio alginolyticus dldh Deletion
• 基金项目: Supported by National Natural Science Foundation of China （No. 31402344） , Natural Science Foundation of Guangdong Province （S2013040014562）.

[Objective] This study aimed to investigate the role of dihydrolipoamide dehydrogenase( DLDH) in the pathopoiesis of Vibrio alginolyticus. [Method] Using suicide plasmid pRE112 as the vector,dldh deletion mutant strain ZJ03Δdldh was constructed with inser