论文部分内容阅读
The effect of penicillin on the human peripheral lymphocytes was studied by steady fluorescent technique and ratiometric fluorescence dye, Fura-2. The change of the free calcium concentration in cytosol was examined under different conditions. A characterization of Fura-2-Ca interaction in an isotonic saline solution showed that Ca2+ formed a 1*1 Fura-2-Ca complex with the apparent dissociation constant 1.81×10-7 mol/L. The mechanism, by which penicillin induced the decrease of [Ca2+]i , was discussed in detail. The low dose of penicillin might modify the lymphocytes’ immunology response by interfering the increase in the intracellular free calcium concentration.
The effect of penicillin on the human peripheral lymphocytes was studied by steady fluorescent technique and ratiometric fluorescence dye, Fura-2. The change of the free calcium concentration in cytosol was examined under different conditions. A characterization of Fura-2-Ca interaction in an isotonic saline solution showed that Ca2 + formed a 1 * 1 Fura-2-Ca complex with the apparent dissociation constant 1.81 × 10-7 mol / L. The mechanism, by which penicillin induced the decrease of [Ca2 +] i was was in detail The low dose of penicillin might modify the lymphocytes’ immunology response by interfering the increase in the intracellular free calcium concentration.