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目的通过观察绒毛组织凋亡发生和胰岛素样生长因子-2(IGF-Ⅱ)表达情况,来探讨稽留流产发生的可能原因。方法分别选择30例无明显诱因导致的稽留流产患者和正常早孕要求人工流产的患者作为研究对象,采用DNA缺口原位末端标记技术(TUNEL法)检测绒毛滋养细胞的凋亡情况;采用SABC法检测绒毛组织中IGF-Ⅱ的表达情况。结果稽留流产组滋养细胞凋亡指数明显高于对照组,前者IGF-Ⅱ的表达明显高于后者。结论滋养细胞过度凋亡及IGF-Ⅱ缺乏可能是导致稽留流产的重要原因。
Objective To explore the possible causes of missed abortion by observing the occurrence of apoptosis in villi and the expression of insulin-like growth factor-2 (IGF-Ⅱ). Methods Totally 30 abortion induced abortion patients and normal abortion requiring abortion were selected as study objects. TUNEL method was used to detect the apoptosis of villous trophoblast cells. SABC method was used to detect The expression of IGF-Ⅱ in villi. Results The apoptosis index of trophoblast cells in missed abortion group was significantly higher than that in control group, the former was significantly higher than the latter. Conclusion The excessive apoptosis of trophoblast cells and the deficiency of IGF-Ⅱ may be the important reasons for missed abortion.