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为探讨酯酶染色技术在鉴别T与非T淋巴细胞上的意义,从正常人(26例)外周血分离淋巴细胞,作酯酶染色,同时与E花结试验作对比,以研究两种技术的相关性。结果如下: 1、99.3±2.1％(均值±标准差)的活性ERFC(E花结形成细胞),94.4±7.2％的总ERFC均呈ANAE(+)。 2、ANAE(+)淋巴细胞中,82.4±5.6％具有形成E花结的能力。 3、总ERFC百分率为60.9±8.5％:ANAE(+)淋巴细胞(12例)百分率为56.5±10.5％,二者的差别不显著(P>0.05)。由上可知,ANAE活性和E花结形式成能为常平行存在于T淋巴细胞,二者均可作为T淋巴细胞的标志,酯酶染色由于其方法简便,如能严格控制实验条件,在细胞免疫测定中将获得广泛应用。
To explore the significance of esterase staining in the identification of T and non-T lymphocytes, lymphocytes were isolated from peripheral blood of normal people (26 cases) for esterase staining and compared with E-flowering test to study two techniques Relevance. The results were as follows: 1,99.3 ± 2.1% (mean ± standard deviation) active ERFC (E-flowered cells) and 94.4 ± 7.2% of total ERFC showed ANAE (+). 2, ANAE (+) lymphocytes, 82.4 ± 5.6% have the ability to form E rosette. The percentage of total ERFC was 60.9 ± 8.5%. The percentage of ANAE (+) lymphocytes (12 cases) was 56.5 ± 10.5%. There was no significant difference between the two groups (P> 0.05). From the above we can see that the ANAE activity and the E-form of flowers can often exist parallel to the T lymphocytes, both of which can be used as a sign of T lymphocytes, esterase staining due to its simple method, such as strict control of experimental conditions, Immunoassay will be widely used.