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目的研究鼻咽癌细胞系ＳＵＮＥ中ＥＢＶ┐ＬＭＰ１基因对上皮细胞增殖的影响，探索ＬＭＰ１在鼻咽癌发生中所起的作用。方法用ＬＭＰ１基因真核表达质粒转染人胚肾上皮细胞，检测ＬＭＰ１的表达，观察细胞在软琼脂中的集落形成能力，ＭＴＴ吸收能力以及ＰＣＮＡ的表达情况。结果被ＬＭＰ１基因转染的细胞生长旺盛，能在软琼脂中形成多个集落，ＭＴＴ吸收能力增强，ＰＣＮＡ的表达水平增高。结论ＬＭＰ１基因能明显改变上皮细胞的生物学行为，促进细胞的生长、增殖和转化，使转染的上皮细胞获得肿瘤细胞的生长特征。
Objective To investigate the effect of EBV-LMP1 gene on epithelial cell proliferation in nasopharyngeal carcinoma cell line SUNE and to explore the role of LMP1 in the pathogenesis of nasopharyngeal carcinoma. Methods The human embryonic kidney epithelial cells were transfected with the LMP1 gene eukaryotic expression plasmid to detect the expression of LMP1. The colony formation ability, MTT absorption capacity and PCNA expression in soft agar were observed. Results The cells transfected with LMP1 gene grew vigorously and could form multiple colonies in soft agar. The ability of MTT absorption increased and the expression of PCNA increased. Conclusion LMP1 gene can significantly change the biological behavior of epithelial cells, promote cell growth, proliferation and transformation, so that the transfected epithelial cells to obtain the growth characteristics of tumor cells.