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应用免疫组织化学（ＬＳＡＢ法）检测９例人骨肉瘤ｒａｓ、ｃ－ｍｙｃ原癌基因和Ｒｂ抑癌基因蛋白产物的表达，研究结果表明：原癌基因ｒａｓ、ｃ－ｍｙｃ的蛋白表达阳性率分别为７８％（７例）、８９％（８例）。两种蛋白表达的强度无显著性差异。在９例骨肉瘤中，两种原癌基因蛋白共同表达有６例，占６７％。抑癌基因Ｒｂ蛋白阴性表达为３例，阴性表达率３３％，且都伴有Ｐ２１和ｃ－ｍｙｃ癌基因蛋白的阳性表达。本文研究结果提示Ｐ２１、ｃ－ｍｙｃ和Ｒｂ癌基因蛋白的异常表达可能在骨肉瘤的发生发展起着一定的作用。
The expression of ras, c-myc proto-oncogene and Rb tumor suppressor gene protein product in 9 cases of osteosarcoma was detected by immunohistochemistry (LSAB method). The results showed that the positive rates of ras and c-myc protein expression were as follows: 78% (7 cases) and 89% (8 cases). There was no significant difference in the intensity of the two protein expressions. In 9 cases of osteosarcoma, two proto-oncogene proteins were co-expressed in 6 cases, accounting for 67%. The tumor suppressor gene Rb protein was negatively expressed in 3 cases, and the negative expression rate was 33%. Both were positively associated with P21 and c-myc oncogene proteins. The results of this study suggest that the abnormal expression of P21, c-myc and Rb oncogene proteins may play a role in the occurrence and development of osteosarcoma.