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首先通过电沉积纳米金,电聚合聚硫堇对玻碳电极表面进行了修饰,之后利用沙丁胺醇为模板分子,通过电聚合α-甲基丙烯酸,制备了一种选择性具有特异识别功能的纳米金/聚硫堇/沙丁胺醇分子印迹电化学传感器。实验采用循环伏安法和差分脉冲法,对不同的修饰电极进行了表征,优化电极的制备和测定条件,研究了印迹传感器对模板分子的选择性响应。结果表明:最佳制备条件下,模板分子:功能单体=1:4,聚合15圈,以0.5 mmol/L硫酸:乙腈(1:9,V/V)洗脱10 min,在pH=7.0的PBS溶液中进行DPV检测,线性范围为2.0×10~(-7)~1.0×10~(-4) mol/L,检测限为6.0×10~(-8) mol/L,S/N=3。该传感器灵敏度高,抗干扰能力强,重现和稳定性好。利用制备的分子印迹电化学传感器对食品中的沙丁胺醇进行了测定,回收率在86.5%~111.9%(n=3),结果令人满意。
Firstly, the surface of glassy carbon electrode was modified by electro-deposition of nano-gold and electropolymerization of polythionine. Then, the surface of the glassy carbon electrode was modified by using albuterol as the template molecule and electropolymerized by α-methacrylic acid to prepare a selective gold nanoparticle / Polythionine / salbutamol molecularly imprinted electrochemical sensor. Cyclic voltammetry and differential pulse methods were used to characterize the different modified electrodes. The preparation and determination conditions of the electrodes were optimized. The selective response of the imprinted sensor to the template was studied. The results showed that the optimal conditions for the preparation of the template were as follows: the molecular weight of the template was 1: 4 and the polymerization time was 15 min. The reaction was performed at pH = 7.0 with 0.5 mmol / L sulfuric acid: acetonitrile (1: 9, V / The detection limit was 6.0 × 10 ~ (-8) mol / L, and the linear range was 2.0 × 10 ~ (-7) ~ 1.0 × 10 ~ (-4) mol / L. The detection limit was S / N = 3. The sensor has high sensitivity, strong anti-interference ability, good reproducibility and stability. Salbutamol was determined in food samples using the prepared molecularly imprinted electrochemical sensor. The recoveries ranged from 86.5% to 111.9% (n = 3), and the results were satisfactory.