感染疟原虫的红细胞突起物形成的逆转

• 作 者: 朱良
• 出 处: 国外医学(寄生虫病分册)
• 发表日期: 1985年05期
• 关键词: 突起物 恶性疟原虫 红细胞 筛选培养 体外培养 变种 恶性疟 疟原虫血症 培养基 亚群

人恶性疟原虫株在培养基中培养可发生某些改变,有突起株(K~+)经长期培养可变为无突起株(K~-)。为了鉴定体外培养是否会引起K~-变种或仅是亚群生长,作者将K~+进行克隆,然后筛选培养K~-细胞,对K~-细胞中的原虫进行再克隆,选出K~+细胞。最终证明在K~+存在一种蛋白质,而K~-是没有的。

Human Plasmodium falciparum strains can undergo some changes when cultured in the medium. The mutant strain (K ~ +) can be transformed into a mutant strain (K ~ -) by long-term culture. In order to identify whether in vitro culture will cause K ~ - variants or only subpopulation growth, the authors cloned K ~ +, and then screened K ~ - cells, K ~ - cells in the protozoa were cloned, select K ~ + Cells. Finally, it is proved that there is a protein in K ~ +, while K ~ - is absent.