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目的: 研究IL-6 影响癌症预后的机制和IL-6与乳腺癌肿瘤相关抗原CA15-3, CEA 和CA125 的关系.方法: 转染外源IL-6基因到MCF-7细胞株, 用酶联免疫吸附法(ELISA) 定量培养液中细胞分泌的CA15-3, CEA和CA125.结果: 在 72 h 培养后, 成功转染pCI-neo-IL-6质粒的MCF-7细胞分泌IL-6 (338.5±22.6) pg/106细胞明显高于没有转染的细胞(25.4±4.6) pg/106细胞 (P＜0.01) 和无IL-6基因的质粒pCI-neo转染的细胞(19.6±3.0) pg/106细胞 (P＜0.01).pCI-neo-IL-6转染的MCF-7细胞分泌CA15-3, CEA 和CA125同样明显高于没有转染的细胞和空载体pCI-neo转染的MCF-7细胞.特异性IL-6抗体降低CA15-3, CEA 和CA125在MCF-7细胞和IL-6 cDNA转染的MCF-7细胞中表达.结论: 转染IL-6基因可增强人乳腺癌细胞肿瘤相关抗原的表达,高IL-6血症的乳腺癌病人预后不良部分原因可能是高IL-6刺激癌细胞表达肿瘤相关抗原.“,”AIM: To investigate the mechanism by which IL-6 is involved in cancer prognosis, and further to demonstrate the relationship between IL-6 and tumor-associated antigens such as CA15-3, CEA and CA125 in breast cancer. METHODS: In the present study, we transfected an exogenous IL-6 gene into the MCF-7 cells. Secretion of CA15-3, CEA and CA125 into the culture media were measured by Enzyme Linked Immunosorbent Assay (ELISA). RESULTS: After a 72 hours in culture, the amount of IL-6 in the media of pCI-neo-IL-6-transfected MCF-7 cells (338.5±22.6 pg/106 cells) was significantly higher than that of non-transfected MCF-7 cells (25.4±4.6 pg/106 cells, P＜0.01, paired t-test), or pCI-neo-transfected MCF-7 cells (19.6±3.0 pg/106 cells, P＜0.01, paired t-test). The levels of CA15-3, CEA and CA125 secreted by the pCI-neo-IL-6-transfected MCF-7 cells were significantly higher than that of the parental MCF-7 cells or pCI-neo-transfected MCF-7 cells. The specific IL-6 antibody could decrease the expression of CA15-3, CEA and CA125 in both the MCF-7 cells and the IL-6 cDNA-transfected MCF-7 cells. CONCLUSION: Transfer of IL-6 gene augments tumor-associated antigens of human breast cancer cells. The association of elevated IL-6 concentration with a poor prognosis in cancer patients may partially be the result of increased expression of tumor-associated antigens by IL-6.