论文部分内容阅读
目的:探讨使用显微外科技术进行自体带血管淋巴结-脂肪组织移植的可行性。方法:18只日本大耳白兔,全麻下采集自体腹股沟区浅层带血管淋巴结-脂肪组织移植于同侧前肢腋窝,利用显微外科技术吻合移植物血管,术后24周连续观察移植物存活情况,比较肢体体积变化。结果:术后16只动物上肢的移植物可以长期稳定存活并具有相应生理功能,供区采集淋巴结-脂肪组织后未诱发下肢慢性淋巴水肿,双下肢体积变化无明显统计学差异,t=1.000,P=0.333。结论:自体淋巴结-脂肪组织移植技术安全、简便、可行,动物实验为该技术向临床应用过渡提供了参考。
Objective: To explore the feasibility of using microsurgical technique for autologous vascular node-adipose tissue transplantation. Methods: Eighteen Japanese white rabbits were collected under general anesthesia. The superficial vessels with vascular lymph node-adipose tissue were transplanted into the armpit of the ipsilateral forelimb under general anesthesia. The grafts were grafted by microsurgical technique and the grafts were continuously observed 24 weeks after operation Survival, changes in limb volume. Results: The grafts of the upper extremities of 16 animals could survive stably for a long time and had corresponding physiological functions. Lymphatic edema of the lower extremities was not induced in the donor area for lymph node - adipose tissue acquisition. There was no significant difference in the volume of the lower extremities between the two groups (t = 1.000, P = 0.333. Conclusion: Autologous lymph node-adipose tissue transplantation is safe, simple and feasible. Animal experiments provide a reference for the transition of this technology to clinical application.