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疟疾酶联免疫吸附试验(ELISA)用的抗原,有以猴的诺氏疟原虫制备的异种抗原和以夜猴感染的恶性疟原虫制备的抗原。本文作者以冰冻保存5年的恶性疟原虫感染夜猴,然后按 Trager 等的培养皿法培养,在疟原虫数增至10～20倍时,以1升容量的组织培养瓶代替培养皿。培养基为30mM 的 He-pes 缓冲液和有10%人血清的 RPMI 1640,培养用的人细胞和人血清均为“O”型。红细胞的浓度为8%,气体的 CO_2和 O_2浓度分
Antigens used in the malaria enzyme-linked immunosorbent assay (ELISA) include: antigens prepared from Plasmodium knowlesnii of Monkeys and heterologous antigens from P. falciparum infected with macaques. The authors infected P. falciparum with frozen P. falciparum for five years and then cultured according to the Trager et al. Culture dish. When the number of malaria parasites increased 10 to 20 times, the culture vessel was replaced with a 1-liter tissue culture flask. He-pes buffer of 30 mM and RPMI 1640 with 10% of human serum were used as the culture medium, and human culture cells and human serum were all in the “O” form. Erythrocyte concentration of 8%, the gas concentration of CO_2 and O_2