论文部分内容阅读
The present study highlights an efficient plant regeneration system in teak(Tectona grandis L.) using forced softwood shoots as an initial plant material. Forced softwood shoots of teak were cut to prepare shoot tip, nodal and internodal explants and cultured on Murashige and Skoog(MS) medium + NAA(1, 3, 6, 10, and 15 μmol · L-1) or TDZ(0.001, 0.01, 0.1, 1, 4, 8, 10, 12 μmol · L-1) for callus induction. Such calluses were further grown on the same levels of TDZ or 0.4, 1, 4, 8, 10 μmol · L-1 BA + 1 μmol · L-1 IBA or GA3. Callus induction was the highest with4.55 cm3 callus volume and 5.75 g dry weight at 0.1 μmol · L-1 TDZ from shoot tips after 35 days. Embryogenic calluses were then shifted to 6, 8 or12 μmol · L-1 TDZ + 2 μmol · L-1 BA or IBA along with 5 mmol · L-1 ascorbic acid(AA) for shoot regeneration from embryogenic cultures. The highest embryogenesis(100%) with 36.4 globular and 5.5 heart-shaped embryo-like structures was obtained at 8 μmol · L-1 TDZ + 2 μmol · L-1 BA after 63 days. Such cultures when further maintained on the same medium up to 150 days resulted in 100% shoot regeneration with 16.4 mean shoots.Shoots were elongated up to 50 mm on agar medium + 8 μmol · L-1 BA + 1 μmol · L-1 GA3. An efficient rooting response(70%) was achieved having4.50 mean number and 49.10 mm root length at 8 μmol · L-1 IBA + 8 μmol · L-1 NAA + 0.1% activated charcoal after 36 days. Rooted shoots were acclimatized in glasshouse, achieving 56.6% plantlet survival.
Forced softwood shoots of teak were cut to prepare shoot tip, nodal and internodal explants and cultured on Murashige and Skoog (Tectona grandis L.) MS) medium + NAA (1, 3, 6, 10, and 15 μmol · L -1) or TDZ (0.001, 0.01, 0.1, 1, 4, 8, 10, 12 μmol · L -1) Callus induction was the highest with 4.55 cm3 callus volume and 5.75 g dry weight at 0.1 μmol · L -1 TDZ from shoot tips after 35 days. Embryogenic calluses were then shifted to 6, 8 or 12 μmol · L -1 TDZ + 2 μmol · L -1 BA or IBA along with 5 mmol · L- L-1 ascorbic acid (AA) for shoot regeneration from embryogenic cultures. The highest embryogenesis (100%) with 36.4 globular and 5.5 heart-shaped embryo-like structures was obtained at 8 μmol · L-1 TDZ + 2 μmol Such cultures when further same up to 150 days resulted in 100% shoot regeneration with 16.4 mean shoots. Shoots were elongated up to 50 mm on agar medium + 8 μmol · L -1 BA + 1 μmol · L-1 GA3. An efficient rooting response (70%) was achieved4.50 mean number and 49.10 mm root length at8 μmol · L -1 IBA +8 μmol · L -1 NAA +0.1% activated Charcoal after 36 days. Rooted shoots were acclimatized in glasshouse, achieving 56.6% plantlet survival.