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为明确黄刺玫的花粉萌发特征,采用花粉离体培养法,研究蔗糖、H3BO4、CaCl2、NAA、GA浓度及培养温度、采集时间、贮藏方式对黄刺玫花粉萌发的影响.结果表明:培养温度、培养基中蔗糖、H3BO4、CaCl2、 NAA、GA浓度均显著影响黄刺玫花粉萌发,一天中不同的采集时间和不同的花粉贮藏方式黄刺玫的花粉萌发率不同.在25℃、15％蔗糖的培养基上萌发效果最好,花粉萌发率达67.51％.基本培养基上分别添加H3BO4 150 mg/L、CaCl2 20 mg/L、NAA 20 mg/L、GA 20 mg/L时花粉萌发率较高.在室温带花、室温离体、0℃～2℃带花、0℃～2℃离体4种条件下贮藏花粉,5 h、10 h后培养,各处理间差异不显著,但在室温下,带花贮藏比离体贮藏效果好;0℃～2℃下,离体贮藏比带花贮藏的萌发率高.花粉于10:00～14:00时段采集较好,12:00时采集萌发率最高.本试验探讨不同条件对黄刺玫花粉萌发的影响,为其杂交育种及理论研究提供依据.“,”In order to clear the pollen germination characteristic of Rosa xanthina, the effects of the concentrations of sucrose, H3BO4, CaCl2, NAA and GA, the cultivation temperature, collecting time and the storage method on pollen germination of Rosa xanthina were studied using pollen culture in vitro method. The results showed that cultivation temperature and the concentrations of sucrose, H3BO4, CaCl2, NAA and GA in the medium had significant influence on pollen germination, and the germination rate was different in different collecting time and different pollen storage methods in one day. The best germination effect was observed on 15％ sucrose medium at 25℃, and the pollen germination rate reached 67.51％. When H3BO4 (150 mg/L), CaCl2 (20 mg/L), NAA (20 mg/L) and GA (20 mg/L) were added to the basic medium respectively, the better pollen germination rate was observed. The germination rates had no significant differences among the pollen that were stored at 4 different conditions of room temperature with flower or in vitro, and at 0℃～2℃ with flower or in vitro and cultivated after 5 h and 10 h. While at room temperature, the better effect was obtained when pollen was stored with flower than in vitro. However, at 0℃～2℃, the higher germination rate was observed when pollen was stored in vitro than with flower. The suitable time for pollen collection was from 10:00 to 14:00, and the highest germination rate was appeared when pollen was collected at 12:00. This experiment discussed the influence of different conditions on pollen germination and would provide basis for the cross breeding and theoretical research of Rosa xanthina.