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目的：探讨成人原发性肾病综合征（ＮＳ） 患者外周血单个核细胞（ＰＢＭＣ） 中ＩＬ１３ｍＲＮＡ 表达及ＩＬ１３ 血浆水平变化。 方法：选择１０ 名健康正常对照者和１６ 名ＮＳ患者。采用反转录多聚酶链反应（ＲＴＰＣＲ） 技术，对ＮＳ患者ＰＢＭＣ中ＩＬ１３ ｍ ＲＮＡ表达量进行分析。同时应用ＩＬ１３ 特异的酶联免疫吸附法（ＥＬＩＳＡ） 测定ＩＬ１３ 血浆水平。 结果：ＮＳ患者ＰＢＭＣ 中ＩＬ１３ ｍＲＮＡ 表达量及血浆ＩＬ１３ 水平均较正常对照组增高（ＩＬ１３ ｍＲＮＡ 表达量为１－２８±０－１３ ｖｓ０－４５±０－１２ ，Ｐ＜ ０－０５ ；ＩＬ１３ 血浆水平为５５－７３ ±１２－７２ ｖｓ２８－５１ ±８－３６ ，Ｐ＜ ０－０５）。蛋白尿伴肉眼血尿组ＮＳ患者的ＩＬ１３ 血浆水平较单纯蛋白尿组降低（６８－９１ ±４－３４ ｖｓ５４－６５ ±２－１１ ，Ｐ＜ ０－０５）。直线相关分析表明，ＩＬ１３ 血浆水平与ＮＳ患者蛋白尿和血尿程度呈负相关，ｒ 分别为－０－５５３ 和－０－７０８，Ｐ均小于０－０５。 结论：ＩＬ１３ 参与ＮＳ分子发病机制，ＩＬ１３ 蛋白质分泌水平相对下降，在ＮＳ患者肾小球硬化发生发展中可能起一定作用。
Objective: To investigate the expression of IL13 mRNA in peripheral blood mononuclear cells (PBMC) and the plasma level of IL13 in adult patients with primary nephrotic syndrome (NS). Methods: Ten healthy controls and 16 NS patients were selected. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to analyze the expression of IL-13 mRNA in PBMC of patients with NS. At the same time the application of IL 13-specific enzyme-linked immunosorbent assay (ELISA) determination of IL 13 plasma levels. Results: The expression of IL-13 mRNA and the level of plasma IL-13 in peripheral blood mononuclear cells from NS patients were higher than those from normal controls (IL-13 mRNA expression was 1-28 ± 0-13 vs 0-45 ± 0-12, P <0-05; IL-13 plasma levels of 55-73 ± 12-72 vs28-51 ± 8-36, P <0-05). Proteinuria with gross hematuria NS patients with plasma levels of 13 compared with simple proteinuria (68-91 ± 4-34 vs54-65 ± 2-11, P <0-05). Linear correlation analysis showed that IL-13 plasma levels and NS patients with proteinuria and hematuria was negatively correlated, r were -0-553 and -0-708, P were less than 0-05. Conclusion: IL 13 participate in the pathogenesis of NS molecules, IL 13 protein secretion relative decline in the development of glomerular sclerosis in NS patients may play a role.