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Objective To obtain the recombinant nonfusion extracellular porcine zona pellucidaprotein 3β (pZP3β) in E.coliMethods By modificated the transition initiation region (TIR) in primers, syntheticnucleotide was gained by PCR. Such gene was cloned into pET-3c vector and trans-formed into E.coli BL21 (DE3)pLysS.Results The recombinant nonfusion extracellular pZP 3 β was expressed in E.coli to10% of total cellular proteins, and identified by the Western blot method.Conclusion Modification of nucleotide without changing amino acid sequences is aneffective means to increase nonfusion expression rate of recombinant proteins, suchas pZP3β in E. coli.
Objective To obtain the recombinant nonfusion extracellular porcine zona pellucidaprotein 3β (pZP3β) in E. coliMethods by modificated the transition initiation region (TIR) in primers, syntheticnucleotide was gained by PCR. Such gene was cloned into pET-3c vector and trans-formed into E. coli BL21 (DE3) pLysS.Results The recombinant nonfusion extracellular pZP 3 beta was expressed in E. coli to 10% of total cellular proteins, and identified by the Western blot method. Contact Modification of nucleotide without changing amino acid sequences is aneffective means to increase nonfusion expression rate of recombinant proteins, suchas pZP3β in E. coli.