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简易蒸汽接种的原理是利用水沸蒸汽,把重量极轻的杂菌及孢子冲掉,在接种物的上空小范围内,形成无菌区进行接种,从而避免杂菌污染,获得接种成功。 1、接种方法:接种操作室提前一天打扫干净,地面洒水,保持清洁湿润,用火炉(电炉)作热源。炉上放一铝锅(铁锅)装入适量的水,把接种钩和镊放入锅内,水沸后煮10分钟取出,然后距水面10厘米架一竿子,放入已灭菌并冷却到25℃左右的菌料瓶(袋)。瓶口向上,这时由二人同时操作,一人开口接种,另一人封口取出接完一锅再放一锅,如此接种毕放置培养室培养。
Simple steam inoculation principle is the use of water boiling steam, the light weight of bacteria and spores washed away in the inoculum over a small area, the formation of sterile areas for inoculation, so as to avoid contamination of bacteria to obtain vaccination success. 1, Inoculation method: Inoculation room a day in advance clean, watering the ground, keep clean and moist, with a stove (electric furnace) as a heat source. Put an aluminum pan on the stove (wok) into the amount of water, the inoculation hooks and tweezers into the pot, boiled water and cook for 10 minutes and then removed, and then 10 cm from the water stand a pole, has been sterilized and cooled To about 25 ℃ bacteria bottle (bag). Bottle up, then operated by two people at the same time, one person inoculation, the other person took a pot followed by a pot and then put a pot, so vaccinated Bi placed cultivation room training.