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把体外受精剩余的未受精卵及前胚,转移到EBS+HIS培养基中培养,每日观察并记录其细胞数及形态,根据他们的发育情况分为四组:①停止发育。至少24小时无细胞分裂证据,认为卵裂停止。记录分裂停止时形态,达到的最多细胞数及授精后培养时间。②未停止分裂前胚。大量前胚伴有与预期发育阶段相适应的细胞数。③α-蝇蕈素处理前胚。为了测定合成蛋白质的转录依赖性,24个前胚在放射标记前,培养在100μl含有选择性RNA多聚酶Ⅱ抑制剂α-蝇蕈素100μg/ml的EBS+BSA培养基中16～24小时。④未受精卵。授精后24小时无原核形成
In vitro fertilization of the remaining unfertilized eggs and pre-embryos, transferred to EBS + HIS culture medium, daily observation and record of the number and morphology of their cells, according to their development is divided into four groups: ① stop development. At least 24 hours without evidence of cell division, that cleft stop. Record the morphology at the time of arrest, the maximum number of cells reached and the culture time after insemination. ② did not stop dividing pre-embryo. A large number of pre-embryos accompanied by the expected developmental stages of the appropriate number of cells. ③ α-fly treatment of pre-embryo. To determine the transcriptional dependence of the synthetic protein, 24 pre-embryos were cultured for 16 to 24 hours in 100 μl of EBS + BSA medium containing 100 μg / ml of the selective RNA polymerase II inhibitor α-mannoid 100 μg / ml prior to radiolabelling. ④ Unfertilized eggs. 24 hours after insemination without prokaryotic formation