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历年来已有很多文献记载肿瘤组织和癌变组织的组织化学观察,但用组织化学方法来观察培养中的细胞,资料比较少,观察培养中的癌瘤细胞则更少。Biesele与Marcuse曾利用组织培养的单层细胞这一有利条件来比较肿瘤细胞与正常细胞内酶的分布。Goldstein观察培养中HeLa细胞的去氧核糖核酸,鉴定细胞株与细胞株间的差别,细胞与细胞间的差别。Smith等用组织化学方法来识别培养中在形态上改变了的细胞相当于组织中的何种细胞。我们选用五种长期培养的癌瘤细胞,两种正常组织的培养和一
Over the years, many literatures have documented the histochemical observation of tumor tissues and cancerous tissues. However, histochemical methods were used to observe the cells in culture with relatively few data, and fewer cancer cells were observed in culture. Biesele and Marcuse once used the advantage of tissue cultured monolayer cells to compare the distribution of enzymes in tumor cells and normal cells. Goldstein observed HeLa cells cultured in the deoxyribonucleic acid, cell lines and cell lines to identify the differences between cell and cell differences. Smith et al. Used histochemical methods to identify which cells in the culture the morphologically altered cells corresponded to in the tissue. We chose five long-term cultured cancer cells, two normal tissue cultures and one