论文部分内容阅读
本研究检测白血病患者骨髓中CYR61、CTGF、VEGF-C、VEGFR-2 mRNA的表达水平,探讨白血病发生、发展、浸润、转移中CYR61、CTGF、VEGF-C、VEGFR-2蛋白的相互作用及其临床意义,寻找新的肿瘤标记物,为白血病的诊治提供新的思路。白血病74例,男38例,女36例,年龄6-77岁,中位年龄45岁。对照组12例,男7例,女5例。年龄16-78岁,中位年龄46岁。采用半定量逆转录聚合酶链反应(RT-PCR)方法测定CYR61、CTGF、VEGF-C、VEGFR-2 mRNA的水平。结果表明,各组初发急性和慢性白血病患者骨髓中CYR61、CTGF、VEGF-C、VEGFR-2 mRNA的表达与对照组相比均显著升高(p<0.05)。急性白血病缓解后CYR61和CTGF mRNA的表达高于对照组(p=0.039,0.025)。CTGF mRNA的表达在B-ALL组表达最高,高于AML、CML、CLL、T-ALL组(p=0.002,0.034,0.002,0.010)。AML组CYR61与CTGF、CYR61与VEGF-C、CTGF与VEGFR-2 mRNA的表达均呈正相关(r=0.452,0.466,0.464;p=0.045,0.038,0.039),CML组CYR61与VEGF-C mRNA的表达呈正相关(r=0.882,p=0.000)。急性白血病伴有髓外浸润者VEGF-C和VEGFR-2 mRNA的表达高于不伴有髓外浸润者(p=0.028,0.047)。AML组VEGF-C mRNA的表达与原始细胞数呈正相关(r=0.418,p=0.034)。结论:CYR61、CTGF及VEGF-C、VEGFR-2在白血病发病中相互作用,促进白血病发展、转移及浸润;且这些因子在不同类型白血病及髓外浸润中的作用存在差异。它们可能成为检测白血病的肿瘤标记物;阻断上述因子有可能阻断肿瘤的生长和转移。
This study was designed to investigate the expression of CYR61, CTGF, VEGF-C and VEGFR-2 mRNA in bone marrow of patients with leukemia and to explore the interaction between CYR61, CTGF, VEGF-C and VEGFR-2 protein in leukemia, development, invasion and metastasis Clinical significance, looking for new tumor markers for the diagnosis and treatment of leukemia provide new ideas. 74 cases of leukemia, 38 males and 36 females, aged 6-77 years, the median age of 45 years. Control group, 12 cases, 7 males and 5 females. Age 16-78 years old, the median age of 46 years old. The levels of CYR61, CTGF, VEGF-C and VEGFR-2 mRNA were determined by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) The results showed that the expression of CYR61, CTGF, VEGF-C and VEGFR-2 mRNA in the bone marrow of patients with acute and chronic leukemia in each group were significantly higher than those in the control group (p <0.05). The expression of CYR61 and CTGF mRNA in acute leukemia after remission was higher than that in control group (p = 0.039,0.025). The expression of CTGF mRNA in B-ALL group was the highest, higher than that in AML, CML, CLL, T-ALL group (p = 0.002,0.034,0.002,0.010). The expression of CYR61 and CTGF, CYR61 and VEGF-C, CTGF and VEGFR-2 mRNA in AML group were positively correlated (r = 0.452,0.466,0.464; p = 0.045,0.038,0.039) Expression was positively correlated (r = 0.882, p = 0.000). The expression of VEGF-C and VEGFR-2 mRNA in acute leukemia with extramedullary infiltration was higher than those without extramedullary infiltration (p = 0.028,0.047). AML group VEGF-C mRNA expression was positively correlated with the number of primitive cells (r = 0.418, p = 0.034). CONCLUSIONS: CYR61, CTGF, VEGF-C and VEGFR-2 interact in the pathogenesis of leukemia and promote the development, metastasis and infiltration of leukemia. The roles of these factors in different types of leukemia and extramedullary infiltration are different. They may become tumor markers for the detection of leukemia; blocking these factors may block tumor growth and metastasis.