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Objective:To observe the feasibility of gene silencing of MAT1 gene by small interference RNA in human pan- creatic cancer cells.Methods:BxPC-3 cells were transfected using chemically synthesized double stranded RNA formulated with liposome.Gene expression of MAT1 was assayed by RT-PCR and Western blot respectively.The cell proliferation as- say was carried out by counting alive cells after Trypan blue exclusion.The cells invasion ability was determined by Boyden chamber model.Results:The MAT1 mRNA and protein expression of BxPC-3 cells were significantly down-regulated by small interference RNA compared with the control groups.The expression of MAT1 mRNA was reduced by 55.2% and 64.3% in 24 h and 48 h respectively(P<0.01).The cell proliferation and invasion ability of BxPC-3 cell were significantly inhibited(P<0.01).Conclusion:The results suggest that gene silencing of MAT1 by siRNA can inhibit the cell proliferation and invasion of BxPC-3 cells,which may be a target in the gene therapy of human pancreatic cancer.
Objective: To observe the feasibility of gene silencing of MAT1 gene by small interference RNA in human pan-creatic cancer cells. Methods: BxPC-3 cells were transfected using using fused double stranded RNA formulated with liposome. Gene expression of MAT1 was assayed by RT -PCR and Western blot respectively. The cell proliferation as- say was carried out by counting alive cells after Trypan blue exclusion. The cells invasion ability was determined by Boyden chamber model. Results: The MAT1 mRNA and protein expression of BxPC-3 cells were significantly down-regulated by small interference RNA compared with the control groups. The expression of MAT1 mRNA was reduced by 55.2% and 64.3% in 24 h and 48 h respectively (P <0.01). The cell proliferation and invasive ability of BxPC-3 Cell were significantly inhibited (P <0.01) .Conclusion: The results suggest that gene silencing of MAT1 by siRNA can inhibit the cell proliferation and invasion of BxPC-3 cells, which may be a target in the gene thera py of human pancreatic cancer.