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目的建立测定保健食品中黄曲霉毒素M1的高效液相色谱-串联质谱法。方法样品经甲醇超声提取,用氰基柱将黄曲霉毒素M1分离,用高效液相色谱-串联质谱进行测定,外标法定量。结果黄曲霉毒素M1标准曲线在0.1~2.0 ng/m L范围内有良好的线性关系,r>0.9998,回收率为77.5%~97.8%,RSD<5.0%,检出限为0.1μg/kg。结论该方法结果准确可靠,可节省样品前处理时间,有利于降低检验成本,适用于保健食品中黄曲霉毒素M1的限量测定。
Objective To establish a method for the determination of aflatoxin M1 in health food by high performance liquid chromatography-tandem mass spectrometry. Methods Samples were extracted by methanol and aflatoxin M1 was separated by cyano-column and determined by high performance liquid chromatography-tandem mass spectrometry with external standard method. Results The standard curve of aflatoxin M1 had a good linearity in the range of 0.1-2.0 ng / mL with r> 0.9998. The recoveries ranged from 77.5% to 97.8%. The RSD was less than 5.0% and the limit of detection was 0.1 μg / kg. Conclusion The method is accurate and reliable, which can save sample pretreatment time, reduce the cost of inspection, and be suitable for the determination of aflatoxin M1 in health food.