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目的观察磷脂酶Ａ_２抑制剂４－二溴苯乙酮（４－ＢＰＢ）对大鼠急性失血性休克复苏后早期肺损伤的保护作用。方法纯种ＳＤ大鼠３６只，随机分为假手术组（对照组）、乳酸林格氏液复苏组（ＬＲ组）、乳酸林格氏液＋４－ＢＰＢ复苏组（保护组）。两复苏组动物放血并维持低血压（ＭＡＰ ４～５．３ｋＰａ） ６０分钟，其后１０分钟内分别输入全部自体抗凝血或同时静注４－ＢＰＢ（２５μｍｏｌ）；２０分钟内输入２倍放血量的乳酸林格氏液，观察６０分钟。实验结束时收集血清、肺组织标本或作支气管肺泡灌洗。双缩脲法测定支气管肺泡灌洗液（ＢＡＬＦ）及血清中蛋白的浓度；放免法检测血清中ＴＮＦａ、ＩＬ－１β、ＩＬ－６及ＩＬ－８水平。光镜下比较观察各组大鼠肺组织形态学改变。结果保护组血清ＴＮＦα水平明显低于 ＬＲ组（Ｐ＜０． ０１），而与对照组无明显差异。三组血清ＩＬ－１β，ＩＬ－６及ＩＬ－８水平除ＬＲ组ＩＬ－６与对照组比较有明显差别外，其余则均无明显差异。保护组肺通透指数与对照组比较无显著性差异，而ＬＲ组明显高于其它两组（Ｐ＜０．０１）。支气管肺泡灌洗液中蛋白含量ＬＲ组明显高于其它两组，保护组明显高于对照组（Ｐ＜０．０１）。各组支气管肺泡灌洗液中细胞数及ＰＭ
Objective To observe the protective effect of 4-dibromoacetophenone (4-BPB), a phospholipase A2 inhibitor, on early lung injury after acute hemorrhagic shock in rats. Methods Thirty-six SD rats were randomly divided into sham-operation group (control group), Ringer’s lactate resuscitation group (LR group) and lactated Ringer’s solution + 4-BPB resuscitation group (protective group). The animals in the two resuscitation groups were bled and maintained with hypotension (MAP 4 ~ 5.3kPa) for 60 minutes. All autologous anticoagulated blood or intravenous infusion of 4-BPB (25μmol) was administered within 10 minutes afterwards. 2 times Blood lactate Ringer’s solution, observed for 60 minutes. At the end of the experiment serum, lung tissue samples or bronchoalveolar lavage were collected. The concentrations of TNFa, IL-1β, IL-6 and IL-8 in bronchoalveolar lavage fluid (BALF) and serum were determined by biuret method. The morphological changes of lungs in each group were observed under light microscope. Results Serum levels of TNFα in the protective group were significantly lower than those in the LR group (P <0.01), but not significantly different from those in the control group. The serum levels of IL-1β, IL-6 and IL-8 in the three groups were significantly different from those in the control group except IL-6 in LR group. There was no significant difference between the protective group and the control group, while the LR group was significantly higher than the other two groups (P <0.01). The protein content of bronchoalveolar lavage fluid LR group was significantly higher than the other two groups, protective group was significantly higher than the control group (P <0.01). The number of bronchoalveolar lavage fluid and PM in each group