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目的 探讨载脂蛋白E基因多态性与血管性痴呆的相互关系并对血管性痴呆 (VaD)与老年性痴呆 (AD)基因型进行比较。方法 采用限制性片段长度多态性分析———多聚酶链式反应法 (RFLP PCR)检测了一组VaD患者ApoE基因多态性并与一组AD患者及一组同龄健康人对照组作了比较。结果 VaD患者中 3/ 4型频度和ε4等位基因频度均明显低于AD患者组 (0 .118比 0 .319,P <0 .0 5 ;0 .0 5 89比 0 .180 9,P <0 .0 1) ,VaD组的 3/ 4型及ε4频度与同龄健康人对照组相比差异无显著性意义。年龄亚组分析显示 :VaD组和AD组在 3/ 4型和ε4频度上的差异主要表现在 6 0～ 6 9岁年龄组 (P <0 .0 0 1)。结论 VaD组ε4频度与健康对照差异无显著性意义 ,而明显低于AD组 ,ε4可能并未参与VaD致病过程 ,而VaD与AD可能存在不同的遗传学发病机制。
Objective To investigate the relationship between apolipoprotein E gene polymorphism and vascular dementia and to compare the vascular dementia (VaD) and senile dementia (AD) genotypes. Methods The polymorphism of ApoE gene in a group of VaD patients was detected by restriction fragment length polymorphism analysis (RFLP PCR) and compared with a group of AD patients and a group of healthy control subjects of same age . Results The frequencies of type 3/4 and ε4 alleles in patients with VaD were significantly lower than those in patients with AD (0.118 vs 0.339, P <0.05; 0.0599 vs 0.1880 , P <0.01). There was no significant difference in the frequency of 3/4 type and ε4 in VaD group compared with healthy controls of the same age group. Age-subgroup analysis showed that the difference of frequency of 3/4 and ε4 between VaD group and AD group was mainly in the age group of 60 to 69 (P <0.01). Conclusion The frequency of ε4 in VaD group is not significantly different from that in healthy controls, but significantly lower than that in AD group. However, ε4 may not be involved in the pathogenesis of VaD. However, there may be different genetic pathogenesis of VaD and AD.