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目的探讨降钙素基因相关肽(CGRP)对内皮素1(ET-1)诱导的大鼠血管平滑肌细胞(VSMCs)表型转化和增殖的作用及其可能机制。方法将原代培养的VSMCs分为对照组(A组)、ET-1组(B组)、ET-1+CGRP组(C组,ET-1预处理24h后共培养24h)和CGRP+ET-1组(D组,CGRP预处理24h后共培养24h)。采用免疫细胞化学染色检测细胞增殖,RT-PCR检测高血压相关基因1(HRG-1)和平滑肌22α(SM22α)mRNA表达。结果与A组相比,B组细胞增殖增加,HRG-1和SM22αmRNA表达下调(P<0.05);而与B、C组相比,D组细胞增殖减少,HRG-1和SM22αmRNA表达上调(P<0.05)。结论 CGRP预处理能明显抑制ET-1诱导的VSMCs表型转化和增殖,可能与HRG-1和SM22α表达上调有关。
Objective To investigate the effect of calcitonin gene related peptide (CGRP) on the phenotype transformation and proliferation of rat vascular smooth muscle cells (VSMCs) induced by endothelin 1 (ET-1) and its possible mechanism. Methods Primary cultured VSMCs were divided into control group (group A), ET-1 group (group B), ET-1 + CGRP group (group C, ET- -1 group (group D, CGRP 24 hours after pretreatment co-culture). Cell proliferation was detected by immunocytochemical staining. The mRNA expressions of HRG-1 and SM22α were detected by RT-PCR. Results Compared with group A, the proliferation of group B was increased and the expression of HRG-1 and SM22α mRNA was down-regulated (P <0.05). Compared with group B and C, the cell proliferation was decreased and the expression of HRG-1 and SM22α mRNA was increased <0.05). Conclusion CGRP pretreatment can significantly inhibit phenotypic transformation and proliferation of VSMCs induced by ET-1, which may be related to the up-regulation of HRG-1 and SM22α expression.