论文部分内容阅读
利用“适配体-目标分子-适配体”的“三明治”夹心方式构建液晶生物传感检测三磷酸腺苷(ATP).将ATP核酸适配体片段作为捕获探针固定在经TEA/DMOAP混合组装膜修饰的玻片基底表面,当ATP存在时,裂开的两部分核酸适配体与ATP结合形成双链结构,有效诱导液晶分子取向发生变化从而引起光学信号的亮度及颜色发生变化,实现对ATP的检测,该方法在ATP浓度为10 nmol/L时仍可观测到明显的光学信号变化.这种“适配体-目标分子-适配体”的“三明治”夹心式液晶生物传感方法具有无需标记,操作简单等特点,在快速检测小分子等物质领域中有广泛的应用前景.
A liquid crystal biosensing assay for adenosine triphosphate (ATP) was constructed using a “sandwich” sandwich of “aptamer-target molecule-aptamer ” The ATP aptamer fragment was immobilized as capture probe on a TEA / When the ATP is present, the split aptamer aptamer binds to ATP to form a double-stranded structure, effectively inducing the change of the orientation of the liquid crystal molecules to cause changes in the brightness and color of the optical signal , The detection of ATP was realized, and this method can still observe the obvious change of optical signal when the ATP concentration is 10 nmol / L. The “sandwich” of “aptamer-target molecule-aptamer” The sandwich liquid crystal biosensing method has the advantages of no marking, simple operation and the like, and has wide application prospect in the field of rapidly detecting small molecules and the like.