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目的建立中药紫萁贯众的含量测定方法。方法用柱色谱法从紫萁贯众中分离紫萁酮,对紫萁酮HPLC含量测定色谱条件进行优选。结果分离得到紫萁酮化学单体作为含量测定对照品。含量测定采用Kromasil C18(4.6 mm×250 mm,5μm)色谱柱;乙腈-0.1%磷酸水(17∶83)为流动相;检测波长为332 nm;流速为1 mL.min-1。紫萁酮进样量在1.62~324 ng内与峰面积线性关系良好,r=1.000 0,平均加样回收率为99.90%,RSD为1.67%。不同产地药材中含量为0.026 7%~0.051 7%。结论首次建立了中药紫萁贯众中紫萁酮的HPLC含量测定方法,该方法能有效控制紫萁贯众药材的质量。
Objective To establish a method for determining the content of Chinese medicine Aster. Methods The purpurin was separated from purpurin by column chromatography, and the chromatographic conditions of HPLC determination of purpurone were optimized. Results were isolated schisandrae chemical monomer as the determination of reference substance. The assay was performed on a Kromasil C18 (4.6 mm × 250 mm, 5 μm) column using acetonitrile-0.1% phosphoric acid water (17:83) as the mobile phase at a detection wavelength of 332 nm and a flow rate of 1 mL.min-1. Paclitaxel had a good linear relationship with peak area within the range of 1.62 ~ 324 ng (r = 1.000 0), with an average recovery of 99.90% and a RSD of 1.67%. The content of medicinal herbs in different areas is 0.026 7% ~ 0.051 7%. Conclusion For the first time, HPLC method for the determination of shikionin in Asiatic root of Chinese traditional medicine is established. The method can effectively control the quality of Aster.