Experimental infection of Z:ZCLA Mongolian gerbils with human hepatitis E virus

• 作 者: Yan Hong Zhuo-Jing He Wei Tao Ting Fu Yan-Kun Wang Yong Chen
• 机 构: Institute of Bioengineering,Zhejiang Academy of Medical Science,
• 出 处: World Journal of Gastroenterology
• 发表日期: 2015年03期
• 关键词: infected moderately immunosorbent weekly spleen fecal assessed pathogenesis alan

AIM:To investigate whether Z:ZCLA Mongolian gerbils are readily susceptible to infection by human hepatitis E virus(HEV).METHODS:Z:ZCLA Mongolian gerbils were infected with a clinical HEV strain isolated from an acutehepati tis E patient,and virus pathogenesis was assessed in this host.Non-infected gerbils served as the control group.Feces samples from gerbils were collected weekly for reverse transcription-nested polymerase chain reaction.Serum anti-HEV Ig G and alanine aminotransferase(ALT) were detected by enzyme linked immunosorbent assay.At sacrifice,each animal’s liver,spleen and kidney were collected for histopathologic examination.RESULTS:HEV-infected gerbils showed fatigue,with histopathological changes observed in the liver,spleen and kidney.HEV RNA was detected in fecal samples taken at day 7 after inoculation and the detectable levels lasted out to day 42 after inoculation.Interestingly,ALT levels were only moderately increased in the HEV-infected animals compared with the noninfected control group.CONCLUSION:Z:ZCLA Mongolian gerbils are susceptible to human HEV.

AIM: To investigate whether Z: ZCLA Mongolian gerbils are readily susceptible to infection by human hepatitis E virus (HEV). METHODS: Z: ZCLA Mongolian gerbils were infected with a clinical HEV strain isolated from an acute hepatius E patient, and virus pathogenesis was assessed in this host. Non-infected gerbils served as the control group. Feces samples from gerbils were collected weekly for reverse transcription-nested polymerase chain reaction. Serum anti-HEV Ig G and alanine aminotransferase (ALT) were detected by enzyme linked immunosorbent assay .At sacrifice, each animal’s liver, spleen and kidney were collected for histopathological examination .RESULTS: HEV-infected gerbils showed fatigue, with histopathological changes observed in the liver, spleen and kidney. HEV RNA was detected in fecal samples taken at day 7 after inoculation and the detectable levels lasted out to day 42 after inoculation.Interestingly, ALT levels were only moderately increased in the HEV-infected animals compared with the noninfected control group.CONCLUSION: Z: ZCLA Mongolian gerbils are susceptible to human HEV.