【摘 要】
：
从RAPD扩增的鳜鱼病毒 (SCV)核酸电泳带中回收了二个片断 ,克隆子pUC19质粒 (称为SCVE36 9和SCVE45 0 )。序列分析表明插入片段分别为 36 9bp和 45 0bp与GenBank序列没有显著
【机 构】
：
中国水产科学研究院珠江水产研究所!广东广州510380,中国水产科学研究院珠江水产研究所!广东广州510380,中国水产科学研究院珠江水产研究所!广东广州510380,中国水产科学研究院珠江水产研究所
论文部分内容阅读
从RAPD扩增的鳜鱼病毒 (SCV)核酸电泳带中回收了二个片断 ,克隆子pUC19质粒 (称为SCVE36 9和SCVE45 0 )。序列分析表明插入片段分别为 36 9bp和 45 0bp与GenBank序列没有显著的同源。根据克隆序列设计两对引物P1/ P2 和P3 /P4 ,在健康鳜鱼、病鳜以及提纯的SCV核酸中进行PCR试验。结果表明 ,P1/P2 组引物在SCV基因组中扩增出特异性核酸片段 ,可作为鳜鱼病毒PCR诊断 ,检测片段为 36 9bp。
Two fragments, the cloned pUC19 plasmid (referred to as SCVE36 9 and SCVE45 0), were recovered from the RAPD amplified catfish virus (SCV) nucleic acid band. Sequence analysis showed that the inserts were 36 9bp and 45 0bp, respectively. There was no significant homology with the GenBank sequence. Two pairs of primers, P1 / P2 and P3 / P4, were designed according to the cloning sequence and PCR assays were performed on healthy anchovy, diseased and purified SCV nucleic acids. The results showed that P1 / P2 primers amplified specific nucleic acid fragments in SCV genome and could be used for PCR diagnosis of anchovy virus with 369bp fragment.
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