论文部分内容阅读
目的探讨缺血预处理对家兔肾缺血再灌注损伤后血清电解质及SOD和MDA的影响及作用机制。方法健康新西兰兔27只,随机分为对照(Control)组、单纯缺血再灌注(IR)组和缺血预处理+缺血再灌注(IPC-IR)组。分别检测缺血再灌注后2h和24h的血清中肌酐(Cr)、尿素氮(BUN)、Na+、K+、Ca2+、Cl-、丙二醛(MDA)和超氧化物歧化酶(SOD)的含量。结果缺血再灌注24h后,IR组和IPC-IR组血清BUN均高于Control组,而血清Cr,IR组显著高于Control组(P≤0.05)。缺血再灌注后,IR组血清Na+显著低于Control组和IPC-IR组(P≤0.05),而血清中K+,IR组显著高于Control组和IPC-IR组(P≤0.05)。血清Ca2+在缺血再灌注2h后,IR组显著低于Control组和IPC-IR组(P≤0.05),但缺血再灌注24h后,IPC-IR组显著高于Control组和IR组(P≤0.05)。血清Cl-在再灌注24h后,IR组显著低于Control组和IPC-IR组(P≤0.05)。肾缺血再灌注2h和24h后,血清MDA,IR组最高、IPC-IR组其次,而Control组最低,血清SOD活性为IPC-IR组最高、Control组其次,而IR组最低。结论缺血预处理可以明显改善缺血再灌注损伤后肾脏的功能,尤其在调节血清电解质平衡方面有更好的作用,其机制与预处理后机体抗氧化能力的增强有关。
Objective To investigate the effect of ischemic preconditioning on serum electrolytes, SOD and MDA in renal ischemia-reperfusion injury in rabbits and its mechanism. Methods Twenty-seven New Zealand white rabbits were randomly divided into Control group, IR group, IPC-IR group and Ischemia-reperfusion group. Serum creatinine (Cr), blood urea nitrogen (BUN), Na +, K +, Ca2 +, Cl-, malondialdehyde (MDA) and superoxide dismutase (SOD) were detected at 2h and 24h after ischemia / . Results Serum BUN in IR group and IPC-IR group was significantly higher than that in Control group after 24h of ischemia-reperfusion, but was significantly higher in Cr and IR group than that in Control group (P≤0.05). After ischemia-reperfusion, serum Na + in IR group was significantly lower than that in Control group and IPC-IR group (P≤0.05), but K + and IR group in serum was significantly higher than that in Control group and IPC-IR group (P≤0.05). Serum Ca2 + levels in IR group were significantly lower than those in Control group and IPC-IR group (P≤0.05) at 2h after ischemia-reperfusion, but were significantly higher in IPC-IR group than those in Control group and IR group ≤0.05). After 24h of reperfusion, serum Cl-IR group was significantly lower than that of Control group and IPC-IR group (P≤0.05). After 2h and 24h of renal ischemia-reperfusion, serum MDA and IR group were the highest, followed by IPC-IR group and lowest in Control group. Serum SOD activity was the highest in IPC-IR group, Control group, followed by IR group. Conclusion Ischemic preconditioning can significantly improve the renal function after ischemia-reperfusion injury, especially in regulating serum electrolyte balance. The mechanism is related to the enhancement of the antioxidant capacity of the body after preconditioning.