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目的 探讨茶中多酚类物质EGCG介导鼻咽癌细胞凋亡的机制以及对EB病毒潜伏膜蛋白 (LMP1)诱导凋亡的干预作用。方法 采用已建立的受四环素调控的LMP1表达的鼻咽癌细胞系 (pTet on LMP1HNE2 ) ,经 0 6mg·L-1Dox和 10 0mg·L-1EGCG处理 2 4h后 ,抽提RNA ,分别与含有细胞凋亡相关基因为主的AtlasapoptosiscDNAexpres sionarray膜杂交。结果 EGCG上调或下调某些与鼻咽癌细胞凋亡相关基因表达。结论 EGCG诱导肿瘤细胞凋亡的作用是多个基因及多条信号转导通路作用的结果 ,并对LMP1诱导鼻咽癌细胞凋亡相关基因表达有一定的干预作用
Objective To investigate the mechanism of EGCG mediated apoptosis in nasopharyngeal carcinoma cells and its effect on apoptosis induced by Epstein-Barr virus latent membrane protein (LMP1). METHODS: The constructed pTet on LMP1HNE2 cells were transfected with pTet on LMP1HNE2 and treated with 0 6 mg · L-1Dox and 10 0 mg · L-1EGCG for 24 h. RNA was extracted and incubated with cells containing cells Apoptosis-related gene-based Atlasapoptosis cDNAexpres sionarray membrane hybridization. Results EGCG up-regulated or down-regulated the expression of some genes related to apoptosis of NPC cells. Conclusions EGCG can induce the apoptosis of tumor cells as a result of multiple genes and multiple signal transduction pathways, and also have certain interference effects on LMP1-induced apoptosis-related gene expression in NPC cells