美国枫香组织培养胚状体的发生

• 作 者: H.E.Sommtr C.L.Brown 胡先菊
• 出 处: 贵州林业科技
• 发表日期: 1983年S1期
• 关键词: 胚状体 组织培养 不定胚 悬浮培养 愈伤组织 固体培养基 培养基成分 完整植株 琼脂培养基 无菌苗

美国枫香(Liquidamber Styraciflua)最初在固体培养基上长出的愈伤组织,移置到液体培养基上悬浮培养时,分化出正常形和畸形两种不定胚;再转移到固体培养基上培养时,从畸形不定胚叶处分化出许多次生不定胚。鱼雷期胚可以发育成正常植株。在琼脂培养基上培养时,也观察到了胚状体的发生。

When the callus of Liquidamber Styraciflua which was originally grown on solid medium was transplanted onto the liquid medium and suspended, the normal and abnormal adventitious embryos were differentiated and transferred to a solid medium , Many adventitious embryos differentiate from deformed embryonic leaves. Torpedo embryos can develop into normal plants. Embryoid bodies were also observed when cultured on agar medium.