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目的：对口腔粘膜损害缘分离的上皮组织作直接免疫荧光检查（ Ｄ Ｉ Ｆ） ，探讨应用该技术诊断天疱疮的可靠性。方法：选择２２ 例口腔寻常型天疱疮，从患者口腔粘膜损害缘分离上皮，制成冰冻切片作为 Ｄ Ｉ Ｆ 的底物，用异硫氰酸荧光素（ Ｆ Ｉ Ｔ Ｃ）标记的兔抗人抗体染色检查，并与同一患者经传统活检取材的粘膜组织（ 含部分粘膜下层的完整粘膜块） 的 Ｄ Ｉ Ｆ 检查作比较。结果：分离上皮的 Ｄ Ｉ Ｆ，２２ 例全部有 Ｉｇ Ｇ 沉积在棘细胞间，并有３ 例 Ｉｇ Ａ、、１ 例 Ｉｇ Ｍ、１３ 例 Ｃ３ 沉积在棘细胞间，与传统活检法的粘膜组织 Ｄ Ｉ Ｆ检查结果相同。结论：从口腔粘膜损害缘分离的上皮组织作 Ｄ Ｉ Ｆ检查，用于诊断天疱疮其结果可靠，操作简便易行，创伤轻微。
OBJECTIVE: To determine the direct immunofluorescence (D I F) of epithelial tissue isolated from the mucosal lesions of the oral mucosa and to evaluate the reliability of the technique in the diagnosis of pemphigus. Methods: Twenty-two cases of pemphigus vulgaris were selected and epithelial cells were isolated from the mucosal lesion edge of the patient. Frozen sections were made as the substrate of D I F. Rabbit anti-FITC labeled Human antibody staining was compared with that of the same patient with conventional biopsy of mucosal tissue (with intact mucosal masses of partial submucosa). RESULTS: All of the 22 cases with IgF deposited in the epithelium had IgG deposited in the spinous cells with 3 cases of IgA, 1 case of IgM and 13 cases of C3 deposited in the spinous cells. Compared with the conventional biopsy mucosa D I F same test result. Conclusion: The epithelial tissue isolated from the mucosal lesion edge of the oral mucosa for the D I F examination for the diagnosis of pemphigus its reliable results, easy to operate, minor trauma.