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目的:研究螺旋藻多糖(PSp)对环磷酰胺和~(60)CO-γ射线所致小白鼠和犬造血系统抑制的影响。方法:腹腔注射环磷酰胺以及用~(60)CO-γ射线照射分别诱发小鼠和犬的骨髓损伤。全血细胞计数和骨髓有核细胞计数。用紫外分光光度计检测骨髓DNA的含量。结果:环磷酰胺和~(60)CO-γ射线分别造成小鼠和犬骨髓造血系统抑制性损伤。PSp 30,60mg/kg能升高小鼠全血白细胞数和骨髓有核细胞数以及DNA含量;PSp 12mg/kg能使犬骨髓有核细胞数,以及外周血红细胞、白细胞及血红蛋白水平得以回升(P<0.01),其疗效优于盐酸小壁胺。结论:PSp对造血系统有化学保护和放射保护作用。
Objective: To study the effect of polysaccharide (PSp) on hematopoietic system inhibition induced by cyclophosphamide and ~(60)CO-γ rays in mice and dogs. METHODS: Intraperitoneal injection of cyclophosphamide and irradiation with ~(60)CO-γ-rays induced bone marrow damage in mice and dogs, respectively. Complete blood counts and bone marrow nucleated cell counts. The content of bone marrow DNA was detected by an ultraviolet spectrophotometer. RESULTS: Cyclophosphamide and ~(60)CO-γ-rays caused the inhibitory damage to bone marrow hematopoietic system in mice and dogs, respectively. PSp 30,60mg/kg can increase the number of whole blood white blood cells and bone marrow nucleated cells and DNA content; PSp 12mg/kg can make the dog bone marrow nucleated cells, and peripheral blood red blood cells, white blood cells and hemoglobin levels can be recovered ( P <0.01), its efficacy is superior to small wall amine hydrochloride. Conclusion: PSp has chemical and radioprotective effects on the hematopoietic system.