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用体外培养法研究ＧＨＭ１０对Ｌ１２１０细胞生长的抑制作用和作用机制。结果表明，Ｌ１２１０细胞在用ＧＨＭ１０处理１ｈ，２４ｈ和７ｄ后，ＩＣ５０依次为６８５，３３２和１５９μｇ·ｍｌ－１，提示ＧＨＭ１０有非时相特异性细胞毒药物的特性。在用ＧＨＭ１０１～２μｇ·ｍｌ－１作用２４ｈ后，Ｌ１２１０细胞的增长率和有丝分裂指数下降，细胞核形态发生变化，但活细胞率仍保持在９６％以上，提示ＧＨＭ１０主要抑制细胞的增殖。用流式细胞计对Ｌ１２１０细胞进行细胞周期动力学的分析表明，ＧＨＭ１０可在一定程度上阻断Ｇ１期细胞向Ｓ期移行，还可增加Ｌ１２１０细胞的膜流动性
In vitro culture method to study GHM 10 inhibition of L1210 cell growth and mechanism of action. The results showed that, L1210 cells treated with GHM 10 1h, 24h and 7d, the IC50 followed by 6 85,3 32 and 1 59μg · ml-1, suggesting that GHM 10 non-phase-specific cytotoxic drugs Features. After treatment with GHM 101 ~ 2μg · ml-1 for 24 h, the growth rate and mitotic index of L1210 cells decreased and the morphology of nucleus changed, but the viable cell ratio remained above 96%, suggesting that GHM10 mainly inhibited cell proliferation . Flow cytometry L1210 cell cycle kinetic analysis showed that GHM 10 to a certain extent, block the G1 phase cells to S phase transition, but also increase the membrane fluidity of L1210 cells