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用粗提的约氏疟裂殖子免疫BALB/c小鼠,取其脾细胞与Sp 2/0瘤细胞融合,获得13株分泌抗约氏疟红内期单克隆抗体的杂交瘤。这些McAb分别属于小鼠:IgG_1,IgG_(2a),IgG_(2b)及IgG_3亚类。据免疫荧光观察,13株McAb可分为4类:1.与红内期各发育阶段的原虫能出现荧光反应;2.针对晚期滋养体及裂殖体;3.抗裂殖体及裂殖子;4.单纯抗裂殖子。有5株McAb与人疟原虫发生荧光反应,其中4株只与恶性疟原虫交叉,M_(26-32)则不仅与恶性疟原虫且与间日疟原虫均有强的交叉反应,,表明约氏疟与人的两种疟原虫间有共同抗原,并提示恶性疟原虫与间日疟原虫间也有共同抗原。共同抗原在不同种间的分布和含量不尽相同。用未经固定的感染红细胞加McAb作间接荧光试验,在感染红细胞表面未观察到荧光反应。
The crude BALB / c mice were immunized with the crude extract of T. yamachii, and the spleen cells were fused with the Sp 2/0 tumor cells to obtain 13 hybridomas secreting the monoclonal antibodies against Plasmodium falciparum. These McAbs belong to mouse: IgG_1, IgG_ (2a), IgG_ (2b) and IgG_3 subclasses, respectively. According to the immunofluorescence observation, 13 McAbs could be divided into 4 groups: 1. Fluorescent reaction could be observed with protozoa in each stage of development in the red stage; 2. For late trophozoites and schizonts; 3. Schizonts and schizonts Child; 4 pure merozoites. Five McAbs reacted fluorescently to P. falciparum, of which four crossed only with P. falciparum. M_ (26-32) not only had strong cross-reactions with Plasmodium falciparum but also with P. vivax, indicating that about Plasmodium and human malaria parasites have common antigens, and also suggested that Plasmodium falciparum and Plasmodium vivax also have common antigen. Common antigens in different species distribution and content vary. Fluorescent reaction was not observed on the surface of infected erythrocytes by indirect fluorescence assay with non-immobilized infected erythrocytes plus McAb.