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目的 流感病毒鸡胚高产母本株A/PR8/34株和X-157株灭活条件的研究.方法 应用改良后的β-丙内酯灭活法对流感病毒鸡胚高产母本株进行灭活.经试验证明,0.5‰β-丙内酯在37℃水浴,pH值范围在7.4-8.0之间条件下作用2h.补加0.5‰β-丙内酯,pH值为9,温度为4℃条件下过夜,能彻底灭活且保证流感病毒表面主要抗原的完整性.结果 采用改良后的β-丙内酯灭活法对流感病毒鸡胚高产母本株A/PR8/34株和X-157株灭活效果良好,鸡胚3代安检合格.灭活前后病毒血凝滴度不变.结论 改良后的β-丙内酯灭活法可用于流感病毒鸡胚高产母本株的灭活.“,”Objective Study on the inactivation condition of β-propiolactone to influenza embryonated egg adapted high yield A/PR8/34 and X-157 strain.Methods Viruses was under 0.5‰ β-Propiolactone,37℃,PH value:7.4-8.0 condition for 2 hour.Then additional 0.5‰ β-Propiolactone was used and change PH value to 9,4℃ overnight.Results The reformed β-propiolactone inactivation method has a good effect on A/PR8/34 and X-157 strain.The HA titer was unchanged before and after activation.Conclusions The reformed β-Propiolactone Inactivation method can be used in the inactivate Influenza embryonated egg adapted high yield strains.