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油桐是重要的木本油料树种,但产量较低,除了栽培因素外,其主要原因是品种低劣。利用杂种优势能有效地进行油桐遗传改良。但因其生育周期长,签定杂种后代需要一定的年限。本研究旨在通过油桐过氧化物酶同工酶的分析,摸索出油桐杂种与亲本同工酶的差异及同工酶与杂种后代表现的关系,为油桐的杂交育种提供依据,这对缩短育种周期有一定价值。 材料与方法 (一)试验材料 采用浙江林学院经济林组培育出来的“五爪桐×少花丛生桐”杂交组合的双亲和F_1代的3个优株(优_1、优_2,杂_4)、一个劣株。采集1—2年生的细根以及叶柄,叶片作为样品。采样的时间为4月22日至5月27日,林龄为8—9年生(“五×少丛”,F_1代4个单株)和15年生(亲本)。 (二)样品处理 野外采集的样品,按不同单株及不同部位,分别编号装入纸袋,立即放入冰壶,拿回实验室,用自来水冲洗
Tung tree is an important woody oilseed species, but the yield is low, except for cultivation factors, the main reason is the poor variety. The use of heterosis can effectively tung tree genetic improvement. However, due to its long fertility cycle, it takes a certain number of years to sign offspring. The aim of this study was to find out the relationship between the isozymes and the isozymes and the progeny of the offspring by analyzing the peroxidase isozymes of the tung tree and provide the basis for the crossbreeding of the tung tree. To shorten the breeding cycle has a certain value. MATERIALS AND METHODS (a) The experimental materials The parents of F1 hybrids of “Wujian tung × lesser flowers tussock” and 3 superior plants of F_1 were cultivated in the economic forest group of Zhejiang Forestry College (excellent_1, excellent_2, 4), a bad strain. Fine roots and petioles of 1-2 years old were collected, and leaves were taken as samples. The sampling time ranged from April 22 to May 27, and the stand age was from 8 to 9 years old (“Five × less plexus”, F 1 generation 4 plants) and 15-year-old (Parents). (B) sample processing Field samples collected, according to different plants and different parts, were numbered into the paper bag, immediately into the curling, back to the laboratory, rinse with tap water