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Ｓ－２－３５淀粉酶抑制剂是一种易溶于水、不溶于有机溶剂的白色粉末，其紫外谱图呈末端吸收，红外谱图在３３９１、１５７２、１４１０、１０３０及９２７ｃｍ￣（－１）处有明显吸收峰。该抑制剂在ｐＨ６．５的条件下对ａ－淀粉酶有明显的抑制作用（ＩＤ＿（５０）为１．７×１０－５ｍｏｌ／Ｌ）．在３７℃，ｐＨ６．５的磷酸缓冲液中，Ｓ－２－３５抑制剂对淀粉酶的抑制作用与抑制剂的浓度关系密切，在抑制剂浓度较小的情况下，抑制剂与酶的结合呈直线关系，随着抑制剂浓度增大，残留活力曲线则出现漂移。根据Ｄｉｘｏｎ图和Ｌｉｎｅｗｅａｖｅｒ－Ｂｕｒｋ图的研究表明，Ｓ－２－３５淀粉酶抑制剂对淀粉酶的结合和底物呈竞争性抑制，抑制常数Ｋ＿ｉ为１．５～１．６×１０￣（－６）ｍｏｌ／Ｌ。
S-2-35 Amylase Inhibitor is a white powder that is soluble in water and insoluble in organic solvents. The UV-Vis spectrum of the S-2-35 is absorbed at the end. The infrared spectrum of the S-2-35 is at 3391, 1572, 1410, 1030 and 927 cm -1 ) At the obvious absorption peak. The inhibitor has a significant inhibitory effect on a-amylase under the condition of pH6.5 (ID_ (50) is 1.7 × 10 -5 mol / L). The inhibitory effect of S-2-35 inhibitor on amylase was closely related to the concentration of inhibitor in phosphate buffer solution of pH6.5 at 37 ℃. When the concentration of inhibitor was low, the binding of inhibitor and enzyme In a linear relationship, as the inhibitor concentration increases, the residual activity curve appears drift. According to the Dixon diagram and the Lineweaver-Burk plot, the S-2-35 amylase inhibitor competitively inhibits the binding of amylase to the substrate, and the inhibition constant K_i is 1.5 to 1.6 × 10 ~ (- 6) mol / L.