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目的建立兔组织笼感染模型并观察左氧氟沙星对金黄色葡萄球菌感染的剂量反应关系。方法在兔背部皮下植入1个无菌高尔夫练习球,待球内充满组织液后,往球内注入金黄色葡萄球菌,建立组织笼感染模型。然后分别给予不同剂量的左氧氟沙星灌胃治疗,抽取组织液测定细菌浓度及耐药性的变化。结果5mg/kg治疗组在治疗过程中细菌浓度降低,治疗结束后细菌恢复生长;10mg/kg和20mg/kg组在治疗后细菌浓度迅速降低,并在治疗过程中细菌恢复生长;而40mg/kg组在治疗过程中细菌浓度迅速降低,在治疗结束后细菌仍未恢复生长。5、10、20mg/kg治疗组都较容易发生耐药,而40mg/kg治疗组无1例耐药发生。结论金黄色葡萄球菌对左氧氟沙星治疗的反应和耐药的发生依赖于左氧氟沙星的剂量。
Objective To establish a rabbit model of caged tissue infection and observe the dose-response relationship of levofloxacin against Staphylococcus aureus infection. Methods A sterile golf practice ball was implanted subcutaneously in the back of rabbits. Staphylococcus aureus was infused into the ball after the ball was filled with tissue fluid, and tissue cage infection model was established. And then were given different doses of levofloxacin gavage treatment, extraction of tissue fluid determination of bacterial concentration and drug resistance changes. Results Bacterial concentration was decreased in the 5 mg / kg treatment group after the treatment, and the bacteria recovered and grew after the treatment. Bacteria concentration decreased rapidly in the 10 mg / kg and 20 mg / kg groups, and the bacteria recovered and grew during the treatment. At 40 mg / kg The bacterial concentration dropped rapidly during the course of treatment, and the bacteria did not recover after the treatment was over. 5,10,20 mg / kg treatment group were more susceptible to drug resistance, and 40 mg / kg treatment group, no case of drug resistance occurred. Conclusion The response of S. aureus to levofloxacin treatment and the occurrence of drug resistance depend on the dose of levofloxacin.