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采用酶联免疫吸附试验(ELISA)法和 ABC 免疫组化法对人卵巢内纤维粘连蛋白(FN)的分布、层粘连蛋白(LN)的分布、FN 与激素的关系进行了观察。结果表明:FN 水平药物诱发排卵的卵泡液(321.3+16.6mg/L)与正常卵泡液(376.9±49.1mg/L)差异无显著性(P>0.05),与同期静脉血清FN(325.0+34.2mg/L)差异亦无显著性(P>0.05),卵泡液 FN 与 E_2水平呈负相关(r=-0.254,P<0.05),FN 与其他激素无相关性(P>0.05)。由此推论:随着卵细胞成熟,E_2增高,FN 降低;FN 与卵泡颗粒细胞分化有关,FN 降低可使卵细胞周围卵丘细胞疏散,有利于卵子成熟与排出。
The distribution of fibronectin (FN), the distribution of laminin (LN), the relationship between FN and hormones in human ovary were observed by enzyme-linked immunosorbent assay (ELISA) and ABC immunohistochemistry. The results showed that there was no significant difference between normal ovarian follicular fluid (321.3 + 16.6mg / L) and normal follicular fluid (376.9 ± 49.1mg / L) (P> 0.05) (P <0.05). The level of FN in follicular fluid was negatively correlated with E 2 (r = -0.254, P <0.05). No correlation was found between FN and other hormones (P> 0.05). Therefore, as the egg matures, E 2 increases and FN decreases. FN is related to follicular granulosa cell differentiation. The decrease of FN leads to the evacuation of cumulus cells around egg cells, which is good for egg maturation and excretion.