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目的观察参附注射液对心源性休克犬血浆NO、iNOS含量及心肌iNOS mRNA的影响。方法选择健康成年家犬15只,体质量(14.0±2.7)kg,雌雄不拘。完全随机分为参附组、模型组,并设假手术组(n=5),在结扎前,休克时,给药后30、60、120、180 min抽血,用比色法测血浆NO、iNOS的含量,180 min时取心肌组织,用RT-PCR法测心肌iNOSmRNA的表达,同时监测犬的心功能指标。结果参附注射液能降低心源性休克时升高的NO[(66.54±2.42)μmol/L vs(45.31±3.39)μmol/L]、iNOS[(6.60±0.23)U/ml vs(5.13±0.23)U/ml]水平(P<0.01),并下调心肌iNOS mRNA的基因表达。参附注射液给药可显著增加心源性休克犬的CO和CI,明显降低动物的TPVR。结论参附注射液可以降低血浆中NO和iNOS的水平,抑制心肌iNOS mRNA的表达。
Objective To observe the effect of Shenfu injection on NO, iNOS and iNOS mRNA in cardiomyocytes of cardiogenic shocked dogs. Methods Fifteen healthy adult dogs were selected and their body weight was (14.0 ± 2.7) kg, both male and female. The rats were randomly divided into three groups: model group, sham-operated group and sham operation group (n = 5). Blood samples were taken at 30, 60, 120 and 180 min after ligation, , And the content of iNOS. The myocardial tissue was taken at 180 min. The expression of iNOS mRNA in myocardium was detected by RT-PCR. At the same time, the canine heart function was monitored. Results Shenfu injection could reduce the NO (66.54 ± 2.42μmol / L vs 45.31 ± 3.39μmol / L), iNOS (6.60 ± 0.23) U / ml vs 5.13 ± 0.23) U / ml] (P <0.01), and down-regulated gene expression of iNOS mRNA in myocardium. Shenfu injection can significantly increase the CO and CI of dogs with cardiogenic shock, significantly reduce the animal’s TPVR. Conclusion Shenfu injection can reduce the level of NO and iNOS in plasma and inhibit the expression of iNOS mRNA in myocardium.