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目的 探讨端粒酶在各种消化道恶性肿瘤中的活性表达情况。方法 利用非放射性的PCR-ELISA反应试剂盒检测76例消化道恶性肿瘤(包括食管癌、胃癌、结肠癌、直肠癌)及10例患者的正常及癌旁组织的端粒酶活性。结果 经过一次PCR-ELISA反应,76例恶性肿瘤中31例呈阳性,正常及癌旁组织均阴性;对一次PCR反应呈阴性的肿瘤组织23例、正常及癌旁组织各10例的PCR产物再次进行PCR-ELISA检测,发现前者有17例反应为强阳性,后两者中仅2例癌旁组织呈强阳性,其余全为阴性;没有发现不同消化道恶性肿瘤之间、不同分化程度之间、不同组织类型之间及不同性别之间端粒酶活性有显著差异。结论 非放射性的PCR-ELISA法检测肿瘤组织端粒酶活性敏感性较低,原因在于肿瘤中存在着PCR反应抑制剂,利用两次PCR可以提高端粒酶活性检出率
Objective To investigate the activity of telomerase in various digestive tract malignancies. Methods The non-radioactive PCR-ELISA kit was used to detect the telomerase activity in 76 normal digestive tract malignant tumors (including esophageal cancer, gastric cancer, colon cancer, rectal cancer) and 10 normal patients. Results After a PCR-ELISA reaction, 31 cases of 76 malignant tumors were positive, normal and paraneoplastic tissues were negative; 23 cases of tumor tissues that were negative for a PCR reaction, and PCR products of 10 cases of normal and adjacent tissues were again A PCR-ELISA test revealed that 17 cases were strongly positive in the former. In the latter two cases, only two adjacent tissues were strongly positive and the rest were all negative; no differences were found between different gastrointestinal malignant tumors and different degrees of differentiation. There were significant differences in telomerase activity between different tissue types and between different sexes. Conclusion Non-radioactive PCR-ELISA method is less sensitive to detection of telomerase activity in tumor tissues. The reason is that PCR inhibitors are present in tumors. Detection of telomerase activity can be improved by using two PCRs.