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目的探讨肺血管内巨噬细胞(PIM)在感染性急性肺损伤(ALI)发病中的作用。方法仿Morton法灌洗肺血管床,贴壁法分离猪PIM,培养于RPM I 1640培养基,予10 mg/L脂多糖(LPS)刺激,胸腺细胞增殖法测PIM培养上清白细胞介素-1β(IL-1β)活性,酶联免疫吸附试验(ELISA)法测肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)含量。结果 LPS刺激后,PIM释放TNF-α、IL-1β和IL-8增多,峰值分别出现在刺激后的1、2和6 h。与刺激前相比,P<0.01。结论 LPS刺激后,PIM分泌多种细胞因子,其中TNFα-、IL-1β升高最早,提示其在ALI发病早期起重要作用;而IL-8升高较晚,且后者持续时间较长,可能对ALI的病情进展起重要作用。细胞因子间的相互作用在ALI的发病中似乎更为重要。
Objective To investigate the role of pulmonary vascular macrophages (PIMs) in the pathogenesis of acute lung injury (ALI). Methods The porcine pulmonary vascular bed was perforated by morton method and the porcine PIM was isolated by adherent method. The porcine PIMs were cultured in RPMI 1640 medium and stimulated with lipopolysaccharide (LPS) at 10 mg / L. The supernatant of interleukin- 1β (IL-1β) activity and the levels of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) by enzyme linked immunosorbent assay (ELISA) Results After LPS stimulation, the release of TNF-α, IL-1β and IL-8 in PIM increased, the peaks appeared at 1, 2 and 6 h after stimulation, respectively. Compared with before stimulation, P <0.01. Conclusions After stimulated by LPS, PIMs can secrete a variety of cytokines, of which TNFα- and IL-1β are the first to be elevated, suggesting that they play an important role in the early stage of ALI. However, IL-8 rises later and the latter lasts longer, It may play an important role in the progression of ALI. The interplay between cytokines appears to be more important in the pathogenesis of ALI.