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作者采用侧翼DNA探针F9(Xq27)和St14-1[DXS52(Xq28)]分析了三个脆性X受累家系。从外周血淋巴细胞中分离出基因组DNA,用限制性内切酶切割DNA分子,利用Southern印迹法研究限制性片段长度多态(RFLP)。并利用LIPED进行两点连锁分析。在所研究家系中的两个家系出现F9基
The authors analyzed three Fragile X-affected families using flanking DNA probes F9 (Xq27) and St14-1 [DXS52 (Xq28)]. Genomic DNA was isolated from peripheral blood lymphocytes, DNA molecules were digested with restriction enzymes, and restriction fragment length polymorphisms (RFLPs) were studied by Southern blotting. And using LIPED for two-point chain analysis. Two of the families in the family studied showed F9 groups