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目的探讨钙磷含量及比例对大鼠肝组织微量元素含量的影响。方法将1月龄清洁级Wistar雄性大鼠随机分成7组,每组10只,饲喂7种不同钙磷含量的试粮,试验期42 d。结果相同磷进食水平下,肝铜、肝锰含量在不同钙磷比之间无显著性差异(P>0.05);肝铁含量随日粮钙水平的提高而显著下降(P<0.05);肝锌、肝镁含量在低钙组与高钙组之间存在显著性差异(P<0.05)。相同钙进食水平下,钙磷比4.0∶1组(低磷)的肝铜含量显著高于其它两组(P<0.05);肝铁、肝锰含量在三组之间存在显著性差异(P<0.05)。相同钙磷比水平下,高钙高磷组的肝铁、锰、镁含量明显低于其它组(P<0.0 5)。结论钙影响肝铁、锌、镁的含量,对铜、锰影响不明显。磷影响肝铜、铁、锰的含量,对锌、镁影响不明显。钙磷比为4.0∶1(Ca 1.00%,P 0.25%)时肝中微量元素含量最高,而钙磷比为1.2∶1(Ca 3.00%,P 2.50%)时肝中微量元素含量最低。
Objective To investigate the effect of calcium and phosphorus content on the content of trace elements in rat liver tissue. Methods One-month-old male Wistar rats were randomly divided into 7 groups with 10 rats in each group, fed with 7 kinds of dietary samples with different contents of calcium and phosphorus. The experimental period was 42 days. Results Under the same phosphorus level, there was no significant difference in the content of liver copper and liver manganese between different calcium and phosphorus ratios (P> 0.05). The content of liver iron decreased significantly with the increase of dietary calcium level (P <0.05) The content of zinc and liver magnesium in the low calcium group and the high calcium group were significantly different (P <0.05). Under the same dietary calcium level, the content of copper in the diet with calcium and phosphorus was lower than that in the other two groups (P <0.05). The content of liver iron and liver manganese in the three groups were significantly different (P <0.05). Under the same level of calcium and phosphorus, the content of liver iron, manganese and magnesium in high-calcium-phosphorus group were significantly lower than those in the other groups (P <0.05). Conclusion Calcium affects the content of liver iron, zinc and magnesium, but has no obvious effect on copper and manganese. Phosphorus affect liver copper, iron, manganese content, zinc, magnesium has no significant effect. The content of trace elements in liver was the highest when the ratio of calcium to phosphate was 4.0:1 (Ca 1.00%, P 0.25%), while the content of trace elements in liver was the lowest when the ratio of calcium to phosphorus was 1.2:1 (Ca 3.00%, P 2.50%).