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[目的]研究肺癌抑癌基因1(TSLC1)对人前列腺癌T3B细胞侵袭、成瘤和转移能力的影响。[方法]将克隆有TSLC1全长cDNA的真核表达载体pCI-TSLC1稳定转染至前列腺癌T3B细胞中。实验组T3B细胞转染pCI-TSLC1质粒,以转染空质粒pCI-neo的T3B细胞为对照组,未加任何处理的T3B细胞为空白组。Transwell法检测体细胞体外侵袭能力,将三组细胞分别以4.0×106/200μl浓度注入裸鼠皮下,观察各组成瘤情况,将三组细胞分别以2.0×106/10μl进行骨原位注射建立骨转移瘤模型,观察各组骨转移率。[结果]与对照组和空白组相比,实验组细胞株细胞体外侵袭能力受到显著抑制(P<0.01);实验组皮下瘤出现明显晚于对照组和空白组,而且瘤体也明显小于对照组和空白组(P<0.01);实验组骨转移率为20%,明显低于对照组(100%)和空白组(100%)(P<0.05)。[结论]TSLC1基因明显抑制T3B细胞的侵袭、成瘤和转移能力。
[Objective] To investigate the effect of lung cancer suppressor gene 1 (TSLC1) on invasion, tumorigenesis and metastasis of human prostate cancer T3B cells. [Method] The eukaryotic expression vector pCI-TSLC1 cloned with TSLC1 full-length cDNA was stably transfected into prostate cancer T3B cells. The experimental group T3B cells transfected pCI-TSLC1 plasmid to transfected empty plasmid pCI-neo T3B cells as a control group, untreated T3B cells as a blank group. Transwell method was used to detect somatic cell invasion in vitro. The three groups of cells were injected subcutaneously into the nude mice at the concentration of 4.0 × 106 / 200μl. The tumor formation of the groups were observed. The three groups of cells were injected with 2.0 × 10 6 / Metastasis model was used to observe the bone metastasis rate in each group. [Results] Compared with the control group and the blank group, the invasion ability of cells in experimental group was significantly inhibited in vitro (P <0.01); the experimental group was significantly later than the control group and the blank group, and the tumor was also significantly smaller than the control group (P <0.01). The bone metastasis rate in experimental group was 20%, which was significantly lower than that in control group (100%) and blank group (100%) (P <0.05). [Conclusion] TSLC1 gene significantly inhibits the invasion, tumorigenesis and metastasis of T3B cells.