论文部分内容阅读
本文采用酶放射分析法对新鲜人脑颞叶组织中５ａ－还原酶同功酶的活性分布进行研究。结果显示：（１）在颞叶脑组织中，５ａ－还原酶１和５ａ－还原酶２主要分布在灰质，其酶活性（５ａ－还原酶１，３３．６±４．５ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝１２；５ａ－还原酶２，１３．８±２．９ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝１１）明显高于分布在白质中的酶活性（５ａ－还原酶１，１４．７±２．０ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝１２，Ｐ＜０．００１；５ａ－还原酶２，５．２±０．９ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝１１，Ｐ＜０，０１）；（２）在灰质中，５ａ－还原酶活性主要来自于５ａ－还原酶１，其酶活性（３４．９±２．５ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝３２）明显高于５ａ－还原酶２（１５．０±２．３ｐｍｏｌ·ｒ_（－１）／ｍｇ蛋白，ｎ＝１８，（Ｐ＜０．００１）；（３）５ａ－还原酶１和５ａ－还原酶２的活性在男女性之间差异不显著，且与年龄无相关关系。
In this paper, enzymatic radiochemical analysis of fresh human brain temporal lobe tissue 5a-reductase isoenzyme activity distribution studies. The results showed that: (1) in the temporal lobe brain tissue, 5a-reductase 1 and 5a-reductase 2 are mainly distributed in the gray matter, the enzyme activity (5a-reductase 1,33.6 ± 4.5pmol · h _ (- 1) / mg protein, n = 12; 5a -reductase 2, 13.8 ± 2.9 pmol · h -1 (mg / l protein, n = 11) was significantly higher than that of the enzyme distributed in white matter (5a- Reductase 1, 14.7 ± 2.0 pmol · h -1 (-1) / mg protein, n = 12, P <0.001; 5a-reductase 2.5.2 ± 0.9 pmol · h -1 / Mg protein, n = 11, P <0.01). (2) In the gray matter, the activity of 5a-reductase mainly came from 5a-reductase 1 and its activity was 34.9 ± 2.5 pmol · h -1 (N = 32) was significantly higher than 5a-reductase 2 (15.0 ± 2.3 pmol · r -1 (-1) / mg protein, n = 18, (. 3) 5a- reductase activity 5a- reductase 1 and 2 between men and women were not significant, and no correlation with age.