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目的 探讨肝炎后肝硬化病人T细胞亚群、细胞因子与机体免疫功能紊乱的关系。方法 用流式细胞仪检测T细胞亚群 ,用免疫酶法对细胞因子分别测定。结果 肝硬化患者血清IL— 2的水平明显低于正常对照组 (3 75± 1 2 5 )pg/ml ,与对照组相比有显著性差异 (P <0 0 5 ) ;血清IL— 6,TNF—α水平均高于对照组 ,分别为 (2 7 2 8± 3 3 5 2 )、(4 7 3 83± 5 2 882 )pg/ml ,与正常对照组相比有非常显著性差异 (P <0 0 0 1)。肝硬化患者外周血淋巴CD4+ 、CD8+ 细胞的绝对数均低于正常对照组 ,分别为 (3 5 0 93 8±15 9 1)、(198 12 5± 10 7 61) / μl ,经统计学处理 ,CD4+ 、CD8+ 与正常人相比均具有非常显著性差异 (P <0 0 0 1)。
Objective To investigate the relationship between T cell subsets, cytokines and immune dysfunction in patients with posthepatitic cirrhosis. Methods T cell subsets were detected by flow cytometry and the cytokines were determined by immunoenzymatic method respectively. Results Serum levels of IL-2 in patients with cirrhosis were significantly lower than those in the control group (3 75 ± 125 pg / ml, P <0.05), serum IL-6, (2 7 2 8 ± 3 3 5 2) and (4 7 3 83 ± 5 2 882) pg / ml, respectively, which were significantly different from the control group ( P <0 0 0 1). The absolute numbers of CD4 + and CD8 + cells in the peripheral blood of patients with cirrhosis were lower than that of the normal control group (35,093.8 ± 15 9 1, (198 12 5 ± 10 7 61) / μl, respectively) , CD4 +, CD8 + were significantly different from normal people (P <0.01).