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目的探讨大肠癌患者外周血微转移的检测及临床意义。方法以CK20非同位素RT．PCR方法，检测54例大肠癌患者术前和术后不同时间外周血中结肠癌细胞。结果54例病人外周血中查出CK20阳性表达32例，阳性率59．63％，CK20阳性检出与Duke’s分期、淋巴转移存在差别有显著性。术后3天内外周血CK20阳性检出较术前多出3例，术后早期短程化疗结束后16例转阴。结论大肠癌在早期就可能已累及全身，外周血中CK20的检测可帮助综合判断疾病恶性程度及患者预后，为指导辅助化疗提供了理论依据。术后早期短程化疗对控制大肠癌微转移具有一定的作用。
Objective To investigate the detection and clinical significance of peripheral blood micrometastasis in patients with colorectal cancer. Method was CK20 non-isotope RT. The PCR method was used to detect colon cancer cells in peripheral blood of 54 patients with colorectal cancer before and after surgery. Results The positive expression of CK20 was found in peripheral blood of 32 patients. The positive rate was 59.63%. There was a significant difference between CK20 positive detection and Duke’s stage and lymph node metastasis. Within 3 days after operation, CK20 positive detection in peripheral blood was more than 3 cases before operation. 16 cases turned negative after early postoperative short-term chemotherapy. Conclusion The colorectal cancer may have been involved in the whole body at an early stage. The detection of CK20 in peripheral blood can help to comprehensively judge the malignancy of the disease and the prognosis of the patient, which provides a theoretical basis for guiding adjuvant chemotherapy. Early postoperative short-term chemotherapy has a certain role in the control of micrometastasis in colorectal cancer.